Yong Zhao
Guangxi University, China
Title: Differentiation of Listeria monocytogenes strains base on multi complete gene sequence
Biography
Biography: Yong Zhao
Abstract
The Gram-positive bacterium Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne infection. A multi complete gene sequencing scheme was developed for accurate, and sensitive subtyping L. monocytogenes. Complete DNA sequences for six virulence genes (prfA, plcA, hly, mpl, actA, and plcB) and two housekeeping genes (prs and ldh) in eight American Type Culture Collection (ATCC) L. monocytogenes strains with seven serotypes were sequenced and analyzed. Results showed multiple DNA sequence alignment identified 8 (prs), 5 (prfA), 8 (plcA), 8 (hly), 8 (mpl),8 (actA), 7 (plcB), and 8 (ldh) allelic types and a total of eight unique sequence types. mpl was the most polymorphic gene and the phylogenetic tree based on its sequence differentiated four groups with high bootstrap values. The mpl and ldh genes appear to be good molecular markers for further epidemiological investigation of L. monocytogenes. A combined virulence gene and the housekeeping genes multi complete DNA sequences scheme will be a promising method enhanced discriminatory power for L. monocytogenes.